Cryobiotica research team presents a new technology and concept, which demonstrate that NIR spectroscopy and Aquaphotomics when applied for differentiation of closely related microorganisms with different phenotypic characteristics provide very accurate, fast and non-invasive identification of probiotic strains based on spectral monitoring data of bacterial growth. Our company was the first to use this innovative method for in-vivo evaluation of lactobacilli and selection of probiotic strains with predefined characteristics.
Since 2012 we have started joint research with Japanese scientist at Kobe University in the Laboratory of Biomeasurements Technology, which lead to the development of the first methods for probiotic bacteria selection, based on Aquaphotomics.
The antimicrobial activity of polyphenol-enriched extracts from industrial plant by-products (strawberry and bilberry press residues and distilled rose petals) against probiotic lactic acid bacteria (Lactobacillus delbrueckii subsp. bulgaricus S10 and S19; Lactobacillus rhamnosus YW and S25; Lactobacillus gasseri S20; Streptococcus thermophilus S13 and S32) was investigated.
The aim of the present work was to select strains from genus Lactobacillus with properties,allowing their inclusion in probiotics and probiotic foods - survival in model conditions ofdigestion, high antimicrobial activity and resistance to some of the applied in medicinal treat-ment antibiotics.
A successful immobilization in chitosan of the lactic acid bacteria Lactobacillus acidophilus A., Lactobacillus helveticus H., Lactobacillus casei subsp. casei C. and Lactobacillus plantarum 226-15 was accomplished and high viable cell counts (∼10<sup>10</sup> cfu/g) in the gel beads were obtained. The resistance of the included in the gel matrix cells to model conditions of digestion was investigated.
Lactobacillus plantarum NBIMCC 2415 resistant to low and high values of pH and pepsin, and to high concentrations of bile salts was selected. It was demonstrated that Lactobacillus plantarum NBIMCC 2415 inhibited the growth of pathogenic microorganisms such as Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Proteus vulgaris G, Salmonella sp., Salmonella abony NTCC 6017, Staphylococcus aureus ATCC 25093, Staphylococcus aureus ATCC 6538 P, Listeria monocytogenes and Listeria monocytogenes I at temperature 15-18°C in the meat products.
The antimicrobial activity of polyphenol-enriched extracts from industrial plant by-products (strawberry and bilberry press residues and distilled rose petals) against probiotic lactic acid bacteria (<i>Lactobacillus delbrueckii </i>subsp. bulgaricus - S10 and S19; Lactobacillus rhamnosus - YW and S25; Lactobacillus gasseri - S20; Streptococcus thermophilus - S13 and S32) was investigated.
All living creatures are made of cells,small membrane-bounded compartments filled with a concentrated aqueous solution of chemicals
Efficient, quick and inexpensive screening methods, which provide rapid, <i>in vivo</i> comprehensive probiotic bacteria evaluation, are highly desired in contemporary microbiology. Aquaphotomics is a novel scientific approach for the exploration of aqueous systems through rapid and comprehensive analysis of water-light interaction as a potential source of information for better understanding of the biological world using spectroscopy.
Development of efficient screening method coupled with cell functionality evaluation is highly needed in contemporary microbiology. The presented novel concept and fast non-destructive method brings in to play the water spectral pattern of the solution as a molecular fingerprint of the cell culture system.
Nowadays a quick and inexpensive method, which allows rapid, in vivo comprehensive probiotic bacteria identification, is needed. To elucidate a new concept to evaluate probiotic bacteria, near infrared spectroscopy with aquaphotomics were applied to monitor the growth of eight <i>Lactobacillus bulgaricus</i> and one <i>Lactobacillus gasseri</i> bacteria strains.
